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primer check!!!


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#1 Bilal Malik

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Posted 24 March 2010 - 10:26 PM

hello everybdy....

i have got a problem regarding primer check....ive got two pair of primers but unfortunately dont knw they are specific for which loci.....can any1 help me how to check that... i was wondering if i could BLAST them..but dont knw how to BLAST it..so is BLSAT the answer..if yes then how to BLAST it...?

#2 Vini

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Posted 24 March 2010 - 10:40 PM

hello everybdy....

i have got a problem regarding primer check....ive got two pair of primers but unfortunately dont knw they are specific for which loci.....can any1 help me how to check that... i was wondering if i could BLAST them..but dont knw how to BLAST it..so is BLSAT the answer..if yes then how to BLAST it...?


Hi, you can use NCBI's primer blast software...

#3 Bilal Malik

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Posted 24 March 2010 - 10:43 PM

dont knw how to use it!!! have tried it :rolleyes:

#4 Vini

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Posted 24 March 2010 - 11:41 PM

:rolleyes: go to primer blast, put in ur forward, reverse primer sequences and the organism, against whose genome, u want to do the BLAST..........whats the problem that u were facing????

#5 Bilal Malik

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Posted 25 March 2010 - 03:44 AM

problem is that i dont knw for which organism these primers actually are. The only thing i knw is the sequence of the primers which is written on the voile...

#6 Bilal Malik

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Posted 25 March 2010 - 03:47 AM

so how can i BLAST it without knowing the organism....

#7 Vini

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Posted 25 March 2010 - 04:21 AM

by default, the organism name is set to human beings, u just remove that and leave the space blank.....




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