1 reply to this topic

[canotto]

Is it feasible to stain for a surface antigen, then fix the cells with methanol and subsequently stain for an intracellular antigen?
I have done with commercial fix & perm solutions, but never with methanol and in the datasheet of the intracellular antibody they say cells should be fixed with this

[Denis Baev]

Ethanol and methanol fixation (for DNA content?) is not compartible with surface staining.