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Surface Staining and Methanol Fixation


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#1 canotto

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Posted 24 March 2010 - 03:06 PM

Is it feasible to stain for a surface antigen, then fix the cells with methanol and subsequently stain for an intracellular antigen?
I have done with commercial fix & perm solutions, but never with methanol and in the datasheet of the intracellular antibody they say cells should be fixed with this

#2 Denis Baev

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Posted 25 January 2011 - 07:13 AM

Ethanol and methanol fixation (for DNA content?) is not compartible with surface staining.




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