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detect miRNAs expression by qPCR


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#1 mirstarter

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Posted 24 March 2010 - 07:45 AM

Hi,
Did anyone here detect miRNAs expression by qPCR? I used the kit from SBI to detect several mature miRNAs expression in several cell lines. I found some of them show single dissociation peak, but some show several peaks. As I have to use mature miRNA sequence as forward primer for qPCR, there is no way to optimize the primer. Can anyone help me with this problem? Thanks a lot. :P

#2 Functional Screens

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Posted 24 March 2010 - 02:19 PM

In general, detecting miRNA by Tagman (ABI) showed higher sensitivity and especially specificity than using SYBR Green (SBI).

#3 mirstarter

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Posted 25 March 2010 - 02:52 PM

Thanks, but tagman is more expensive than sybgreen.




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