Hi Friends,
I have a doubt regarding the similarity search of the n-terminal sequence. I have done N-terminal sequencing of my protein of my interest and to find out the similar proteins to this I ran BLAST. But the result is very confusing. B'coz, only just 6-7 proteins show identity? will it be sufficient? or is it necessary that the entire stretch have to match? Please help.
Thank you in advance
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#2
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Posted 25 March 2010 - 04:47 PM
How many residues of sequence data did you get? What is the quality of the data like?
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#3
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Posted 29 March 2010 - 12:32 AM
swanny, on Mar 25 2010, 05:47 PM, said:
How many residues of sequence data did you get? What is the quality of the data like?
15 residues and quality is good
