Therefore I would like to know, if anyone can help me with a protocol of DC (Dendritic-Cell) Isolation from spleen or even of other organs.
Thank you
Posted 13 March 2002 - 10:18 AM
Thank you
Posted 08 April 2002 - 11:45 AM
Posted 20 April 2004 - 07:35 AM
Posted 01 June 2004 - 12:16 PM
Posted 26 July 2004 - 12:20 AM
For this purpose U can isolate Bone marrow cells from femur bone of mouse with pushing and then culture them.My boss asked me to begin to work with IMMATURE dendritic cells but I never isolated dentritic cells and I dont know anything about this technic (usually I am doing proteomics, I did not touch a mouse of my whole life!!!). Do you have a protocol to isolate dc from mouse femur bone marrow , and how to grow them? I have a very poor background in immunology so anything you can tell me will be helpfull.
Thank you very much
Posted 04 October 2004 - 07:31 AM
Edited by Nexus, 04 October 2004 - 08:56 AM.
Posted 12 October 2004 - 10:00 AM
Posted 31 October 2004 - 12:45 AM
how many workers on dendritic cell believe that thsese are nonadherent cells only , i think in presence of GMCSF and IL4 many cell remains attached which shows almost all charecetrs of dendritic cells and not exclusively the morphology is criteria for calling them DCs ; immature DCs never shows dendritic morphology only full blown DCs shows dendritic structure and blunt dendrities ;how many workers on dendritic cell believe that thsese are nonadherent cells only , i think in presence of GMCSF and IL4 many cell remains attached which shows almost all charecetrs of dendritic cells and not exclusively the morphology is criteria for calling them DCs ; immature DCs never shows dendritic morphology only full blown DCs shows dendritic structure and blunt dendrities ;
i have tried to generate DCs from human , rat, mice hamester, guinea pig and rabbit , infact different species DCs donot response same for their differentiation toward immature DCs, many of guyinea pig PBMC even donot survive in the same titre of GMCSF which allows mice and other species PBMCs to direct toward DCs , i want the open answer for this do u all believe the same ; u can try also,,,
only beauty og bone marrow DCs is that it gives an synchronised homogeneous DCs for experiments becs DCs isolated from animals direct with markers like CD11c and OX-62 can not differentiate between immature an dmature stage which is the crucial most aspect of studying now a days ( am i right ? )
Posted 25 November 2004 - 09:43 PM
Posted 13 January 2005 - 11:01 AM
Posted 31 January 2005 - 12:21 PM
Posted 18 February 2005 - 03:25 PM
Posted 23 March 2005 - 04:00 AM
Posted 21 June 2009 - 09:23 PM
Does any one have a good idea of how many DCs should be recovered from a normal mouse spleen?
Posted 22 June 2009 - 07:38 AM
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