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[tantao]

Dear all,
I used the protocol to do the frozen sections as follow:
1.Fixed in formalin for 48h

2.       Immerge in 10% sucrose for 2h;

3.       Immerge in 20% sucrose for 2h;

4.       Immerge in 30% sucrose until the tissue drop down to bottom;

5.       Immerge in 30% sucrose blended with OCT (50%:50%) for 1h;

6.       Immerge in OCT for 1h;

7.       make cryosection in 10um.


However, I used dd water to prepare the sucrose solutions instead of PBS. I really want to know if  it will severely affect my sample.Thanks a lot

Edited by tantao, 24 March 2010 - 02:30 AM.