1. Qiaquick and elute in 30 ul
2. MinElute and elute in 10 ul, then dilute to 30 ul
Both the Qiaquick and MinElute kits state the same predicted yields at ~80%. The protocols for the kits discuss the upper binding capacity of the columns, but not the loss at lower DNA amounts. I need more than the 10 ul elution volume of the MinElute kit, as I will need to perform several qPCR reactions from each DNA sample.
Edited by jamessmith01, 23 March 2010 - 03:40 AM.