3 replies to this topic

[rocketfan86]

Hi all. I am wondering if there is such a way to have a PTM 'permanent' on a protein?

I am wanting to look at APE1 acetylation and is it possible to generate the acetylation to where it cannot be removed?

Thanks in advance.

[laurequillo]

rocketfan86, on Mar 22 2010, 07:54 PM, said:

Hi all. I am wondering if there is such a way to have a PTM 'permanent' on a protein?

I am wanting to look at APE1 acetylation and is it possible to generate the acetylation to where it cannot be removed?

Thanks in advance.

Well, you can create a mutant mimicking an constitutive acetylation status. If you know the lysine/s that are acetylated you can change them from K to Q

[rocketfan86]

laurequillo, on Mar 23 2010, 08:15 AM, said:

rocketfan86, on Mar 22 2010, 07:54 PM, said:

Hi all. I am wondering if there is such a way to have a PTM 'permanent' on a protein?

I am wanting to look at APE1 acetylation and is it possible to generate the acetylation to where it cannot be removed?

Thanks in advance.

Well, you can create a mutant mimicking an constitutive acetylation status. If you know the lysine/s that are acetylated you can change them from K to Q

Hi there. Can you explain how glutamine acts as an acetylation mark? Thanks.

[laurequillo]

rocketfan86, on Mar 23 2010, 09:58 PM, said:

laurequillo, on Mar 23 2010, 08:15 AM, said:

rocketfan86, on Mar 22 2010, 07:54 PM, said:

Hi all. I am wondering if there is such a way to have a PTM 'permanent' on a protein?

I am wanting to look at APE1 acetylation and is it possible to generate the acetylation to where it cannot be removed?

Thanks in advance.

Well, you can create a mutant mimicking an constitutive acetylation status. If you know the lysine/s that are acetylated you can change them from K to Q

Hi there. Can you explain how glutamine acts as an acetylation mark? Thanks.

It mimicks an Ac-K. It is the classical way to check the function of an acetylation site. You change the K to A/R to create a non-acetylable mutant and you change K to Q to create the Acetyl-mimic mutant. It is similar that the mutations to create phospho mutants or phospho-mimic mutants.