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Non-deacetylable Protein?


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#1 rocketfan86

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Posted 22 March 2010 - 10:54 AM

Hi all. I am wondering if there is such a way to have a PTM 'permanent' on a protein?

I am wanting to look at APE1 acetylation and is it possible to generate the acetylation to where it cannot be removed?

Thanks in advance.

#2 laurequillo

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Posted 23 March 2010 - 05:15 AM

Hi all. I am wondering if there is such a way to have a PTM 'permanent' on a protein?

I am wanting to look at APE1 acetylation and is it possible to generate the acetylation to where it cannot be removed?

Thanks in advance.


Well, you can create a mutant mimicking an constitutive acetylation status. If you know the lysine/s that are acetylated you can change them from K to Q
"He must be very ignorant for he answers every question he is asked" Voltaire

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#3 rocketfan86

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Posted 23 March 2010 - 12:58 PM

Hi all. I am wondering if there is such a way to have a PTM 'permanent' on a protein?

I am wanting to look at APE1 acetylation and is it possible to generate the acetylation to where it cannot be removed?

Thanks in advance.


Well, you can create a mutant mimicking an constitutive acetylation status. If you know the lysine/s that are acetylated you can change them from K to Q


Hi there. Can you explain how glutamine acts as an acetylation mark? Thanks.

#4 laurequillo

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Posted 24 March 2010 - 02:25 AM

Hi all. I am wondering if there is such a way to have a PTM 'permanent' on a protein?

I am wanting to look at APE1 acetylation and is it possible to generate the acetylation to where it cannot be removed?

Thanks in advance.


Well, you can create a mutant mimicking an constitutive acetylation status. If you know the lysine/s that are acetylated you can change them from K to Q


Hi there. Can you explain how glutamine acts as an acetylation mark? Thanks.


It mimicks an Ac-K. It is the classical way to check the function of an acetylation site. You change the K to A/R to create a non-acetylable mutant and you change K to Q to create the Acetyl-mimic mutant. It is similar that the mutations to create phospho mutants or phospho-mimic mutants.
"He must be very ignorant for he answers every question he is asked" Voltaire

"This is SPARTA!"

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