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Neutrophil isolation


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#1 bknm

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Posted 22 March 2010 - 09:40 AM

Hi,

I was wondering does anyone know if it possible to isolate neutrophils and PBMCs simultaneously from whole blood? So far I have only been isolating PBMCs using Ficoll.

Thanks.

#2 klinmed

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Posted 22 March 2010 - 11:16 AM

Hi,

I was wondering does anyone know if it possible to isolate neutrophils and PBMCs simultaneously from whole blood? So far I have only been isolating PBMCs using Ficoll.

Thanks.

This is usually fairly straightforward using a single density centrifugation. A number of products are commercially available. For example:

Hope this helps

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