1 reply to this topic

[bknm]

Hi,

I was wondering does anyone know if it possible to isolate neutrophils and PBMCs simultaneously from whole blood? So far I have only been isolating PBMCs using Ficoll.

Thanks.

[klinmed]

bourkenm, on Mar 22 2010, 06:40 PM, said:

Hi,

I was wondering does anyone know if it possible to isolate neutrophils and PBMCs simultaneously from whole blood? So far I have only been isolating PBMCs using Ficoll.

Thanks.

This is usually fairly straightforward using a single density centrifugation. A number of products are commercially available.  For example:

Hope this helps

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