Anybody did any AscI ligation?
Posted 22 March 2010 - 06:05 AM
My vector is 13kb(only have AscI and PacI sites),my products is about 4kb.
I have done the ligation several times with no success.
I suspect it becauses the AscI has a too long over-hang,so what do you think?
Posted 24 March 2010 - 11:27 AM
Posted 05 April 2010 - 05:41 AM
by the way,my vector is 13kb and insert 4kb..
Posted 06 April 2010 - 06:18 AM