2 replies to this topic

[Tai]

Hi

I have human fibroblast and human glutathione deficient cell line ... I'm looking to measure Glutathione synthetase enzme activity.
Could anyone give me some suggestion if anyway to measure ?
what I found on googld mostly for GST method ... i'm not sure if it the same..

[klinmed]

Tai, on Mar 22 2010, 10:48 AM, said:

Hi

I have human fibroblast and human glutathione deficient cell line ... I'm looking to measure Glutathione synthetase enzme activity.
Could anyone give me some suggestion if anyway to measure ?
what I found on googld mostly for GST method ... i'm not sure if it the same..

Glutathione S-transferase (GST) and GS are definitely not the same.
The attached paper may give you some ideas for measuring GS in cell extracts.

Hope this helps

Attached Files

•  GS_assay.pdf   72.15K   106 downloads

[Tai]

thanks klinmed, this is the great help, I found some way to do now