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What are "Ella-cre" mice?


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#1 seriousperson

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Posted 21 March 2010 - 12:45 PM

Hi

I'm reading an article where they engineer knockin transgenic mice.

At one step they inserted a DNA sequence containing Neomycin-resistance into a replacement vector. Then they injected this knockin vector to embryonic stem cells, then they injected these into mouse blastocyst. Fine so far.

Then, they cross-bred these new mice with Ella-cre mice in order to get rid of the Neo-resistance cassette.

OK, I understand this but I want to know what are these "Ella-cre" mice? I searched around and couldn't find.

Thanks

#2 bob1

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Posted 21 March 2010 - 03:40 PM

have a look for cre-lox system.

#3 Rsm

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Posted 22 March 2010 - 12:25 AM

E2A-Cre mouse express Cre from oozyte stage onwards, and in all cell types. It is thus used to achieve recombination in all cells (Deleter-Cre).

Cheers,
Minna
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