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high con of auxin for callus but why shoot develope???


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#1 noor albayan

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Posted 21 March 2010 - 07:34 AM

Hi every one


the story that


I was do experiment and I used media contain different concentration of auxin ( 15mg/l) and 30mg/l . I used that to study its effect on Peanut embryo

I expected to get callus
but actually what happened is that I got shoot when I cultured the epicotyl




I want to know the reasons:???

#2 pito

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Posted 21 March 2010 - 09:15 AM

Dont you need both auxine and cytokines to get callus?

I remember that auxin was for the growth of roots, cytokines for shoots and both for callus.

But I'll have to check my papers for that because its been a while since I had any plantscience

And I also remember that not all auxins would induce callusgrowth

Edited by pito, 21 March 2010 - 09:17 AM.

If you don't know it, then ask it! Better to ask and look foolish to some than not ask and stay stupid.





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