Hi all,
I was trying to proliferate my CD34 cells for the last 3 weeks but the cells didn't grow, I am using HPGM media and different concentration of cytokines 50-100 ng/ml of SCF, TPO and FLT3, with different cell count 20,000-200,00.
Any recommendation?
Thank you in advance
0
1 reply to this topic
#2
-
- Active Members
-
- 42 posts
Enthusiast
Posted 20 July 2010 - 08:27 AM
What is the origin of your cells? i.e. Are they human, mouse...? How did you isolate them?
http://www.stemcell.com/ has some good stuff on there for stem cell culture such as the SFEM.
http://www.stemcell.com/ has some good stuff on there for stem cell culture such as the SFEM.
