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CD 34 proliferation


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#1 Salem

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Posted 21 March 2010 - 04:12 AM

Hi all,
I was trying to proliferate my CD34 cells for the last 3 weeks but the cells didn't grow, I am using HPGM media and different concentration of cytokines 50-100 ng/ml of SCF, TPO and FLT3, with different cell count 20,000-200,00.
Any recommendation?
Thank you in advance

#2 Piersgb

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Posted 20 July 2010 - 08:27 AM

What is the origin of your cells? i.e. Are they human, mouse...? How did you isolate them?

http://www.stemcell.com/ has some good stuff on there for stem cell culture such as the SFEM.




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