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How many miRNA will be down regulated after knocking down Dicer in mouse


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#1 biolearner

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Posted 20 March 2010 - 07:39 AM

We knocked down Dicer in mouse germ cells and found only a small set of microRNA down regulated. Is it reasonable? How about genes? We also got a small set of genes up regulated.

By the way, we detected all mouse microRNA expression by microarray.

Thanks.

#2 miRNA man

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Posted 22 March 2010 - 05:10 AM

How long after siRNA transfection did you check for miRNAs? I think that they can be quite stable.

#3 pcrman

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Posted 23 March 2010 - 02:49 PM

Keep in mind that dicer may be redundant in its function, although dicer knockout in mice is lethal.

#4 Functional Screens

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Posted 24 March 2010 - 02:23 PM

Can competition and saturation explain what you see?

Also, please note you may not be able to "efficiently" knock down Dicer by "shRNA" since shRNA needs Dicer to get processed.




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