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[mirstarter]

When I try to use RNA22 to predict the targets of interesed miRNA, I found there are two setting up: Minimum number of paired-up in heteroduplex, maximum folding energy for heteroduplex(kcal/mal). Is there anyone who can teach me how should I set up the condition. the default value is 14 (Minimum number of paired-up in heteroduplex), -25(maximum folding energy for heteroduplex(kcal/mal). Is there any paper to explain these? Thanks in advance.