how to make 3'UTR reporter
Posted 19 March 2010 - 06:27 AM
Posted 19 March 2010 - 06:59 AM
Posted 19 March 2010 - 04:08 PM
I have found this site used the reporter assay to validate miRNA expression, it's more like miRNA serves as siRNA assay.
If you want to examine a miRNA target messenger RNA, then you might want to clone the 3'UTR of the target gene into a reporter vector.