Posted 18 March 2010 - 06:55 PM
I have a question about mega preps. I am doing a mega prep on my plasmid with the Qiagen kit. In the last step, it says to elute the DNA in a suitable volume of TE buffer. My question is what is that suitable volume? I usually elute in 500 ul and I am not sure if that is too high or too low.
Posted 23 March 2010 - 08:42 AM
Posted 23 March 2010 - 08:47 AM
And in order to know that you need to do some testing.
I used 30Ál as elution total and I used ultra pure MQ and not the buffer
Posted 23 March 2010 - 11:01 AM
2.5 mg / 30 ul => 83 ug/ul
2.5 mg / 500 => 5 ug / ul
Choose whatever you like.
Posted 24 March 2010 - 02:10 AM
sorry for the mistake.
You are 100% correct Vladooo
Posted 24 March 2010 - 01:48 PM