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preparation of solutions.


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7 replies to this topic

#1 nightingale

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Posted 18 March 2010 - 07:50 AM

i was supposed to dissolve 25g of glucose in 100 ml,
but unfortunately,, i got out of d.H2O ...
when i had already started to dissolve the glucose ...
and i have to wait to the second day to have d.H2O.

so i refrigerated the un-known amount of the solution i have,,
and the second day i added d.H2O to reach 100ml..
- it was somewhat 10 ml - ...
and mixed it verrrry well.

is my preparation REALLY a 25g in 100 ml ???
" The more you learn, the more you realize how little you know ... "

#2 Vini

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Posted 18 March 2010 - 07:59 AM

i was supposed to dissolve 25g of glucose in 100 ml,
but unfortunately,, i got out of d.H2O ...
when i had already started to dissolve the glucose ...
and i have to wait to the second day to have d.H2O.

so i refrigerated the un-known amount of the solution i have,,
and the second day i added d.H2O to reach 100ml..
- it was somewhat 10 ml - ...
and mixed it verrrry well.

is my preparation REALLY a 25g in 100 ml ???



hey, I am sorry, but your question is not clear to me.....did the 25g glucose that you took, dissolve in whatever x volume of d.H2O you added the previous day???? even if you made up the volume to 100mL the next day, why are you doubtful about the concentration???

#3 nightingale

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Posted 18 March 2010 - 08:12 AM

thanx for passing DRN :lol:

sorry for this ... :lol:
am ready for any clarification ...

okay to start answering :-
1- did the glc dissolved ?
yes, it did.
2- mmmm,,, because it was already dissolved in the "X" amount and then i made up the volume to 100 ml.

just a thought ...

Edited by nightingale, 18 March 2010 - 08:12 AM.

" The more you learn, the more you realize how little you know ... "

#4 Vini

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Posted 18 March 2010 - 08:16 AM

thanx for passing DRN :lol:

sorry for this ... :lol:
am ready for any clarification ...

okay to start answering :-
1- did the glc dissolved ?
yes, it did.
2- mmmm,,, because it was already dissolved in the "X" amount and then i made up the volume to 100 ml.

just a thought ...


ok Nightingale, now I get your doubt.....It is ok even if you made up the volume later on.....no problem at all with the concentration.... ;)

#5 nightingale

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Posted 18 March 2010 - 03:50 PM

thanks :)
" The more you learn, the more you realize how little you know ... "

#6 Prep!

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Posted 19 March 2010 - 03:08 AM

i know this comes late but still just adding to it!!!!

it wud be a problem only is u have used up some volume.. then the conc will change... otherwise if its not volatile.. which is not in ur case... even if u adjust the volume after a couple of days it s fine!!!!

But a lesson to learn from here is that be ready with everything before doing the experiment!!!! :D
i ve faced some serious problems due to this in the regard so taking the liberty!!! :)

Edited by Pradeep Iyer, 19 March 2010 - 03:13 AM.

Support bacteria - They are the only culture some people have!!!
Cheers!!!

#7 mdfenko

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Posted 19 March 2010 - 07:40 AM

there is no problem if you were supposed to make 25% w/v glucose (25 gm in 100 ml final volume).

it is a problem if you were supposed to add 25 gm to 100 ml water.
talent does what it can
genius does what it must
i do what i get paid to do

#8 nightingale

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Posted 19 March 2010 - 03:43 PM

grateful to ur advice Pradeep Iyer :P
in times i think that no matter how well-prepared you were,,
u have to have such a day ... ;) :P :)

always grateful to you mdfenko, and to your precious notes :)
" The more you learn, the more you realize how little you know ... "




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