Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Protein elution from amylose resin


  • Please log in to reply
1 reply to this topic

#1 spellberg56

spellberg56

    member

  • Active Members
  • Pip
  • 13 posts
0
Neutral

Posted 18 March 2010 - 07:08 AM

Hello, I have my protein of interest bound to Maltose Binding Protein (MBP). I have bound this fusion to amylose resin in a 1.5 ml microfuge tube. I have already tested my amylose bound samples to ensure they are present. The protocol says to elute with 10mM maltose in buffer but the protocol also uses a amylose column, not a microfuge tube. If I add the maltose to the amylose bound resin, do I remove the supernatant to get my eluted protein after spinning down? I won't be able to take fractions when using a micorfuge tube.

Thank you

#2 mdfenko

mdfenko

    an elder

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 2,805 posts
133
Excellent

Posted 19 March 2010 - 07:36 AM

you are performing a batch elution. no problem, just make sure that you thoroughly wash the resin before you elute or your product will be contaminated with material that didn't bind.
talent does what it can
genius does what it must
i do what i get paid to do




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.