Illumina - Bead studio Micrarray DATA
Posted 18 March 2010 - 04:46 AM
Is any one working with bead studio software to analyze the illumina microarray for microRNA differential expression? Got some questions to be answered. Please let me know.
Thanks in advance.
Posted 08 April 2010 - 03:52 AM
Posted 10 April 2010 - 06:30 AM
I am wondering how to compare 4 samples at a time like what we do in ANOVA (ex: A vs B, A vs C, A vs.D B vs. C, B vs. D & C vs. D). Instead the beadstudio software runs a t-test comparing each sample (say B,C,D) with a reference sample (say A).
My other question is: At what fold change can we say that the differential expression of mutant type is significantly different from that of the wild type. (ex: 2x or 3x or 10x).
3rd question: what is the difference between detection p-value and diff. p-value.