I sonicated for at 25% amplitude using 10 second pulses with 30 seconds rest on ice for:
Lane 1 - 6 rounds
Lane 2 - 10 rounds
Lane 3 - 15 rounds
Lane 4 - 20 rounds
I am using the Magnify Chip kit and purifying the DNA using the magnetic beads with the kit.
Personally I think it looks pretty good, it could maybe do with 25 - 30 rounds but then I think it might be overdone a little. I could also increase the duration of each pulse and probably reduce the rounds, but at some point you have to be happy with what you have. Any comments?
Edited by Dukey, 17 March 2010 - 03:09 PM.