Need help with Caspase WB
Posted 17 March 2010 - 02:17 PM
Posted 29 March 2010 - 08:24 AM
do you mean you do not see any cleavage or you do no see anything at all?
If you don't see any caspase3 cleavage then I doubt apoptosis is occuring!
maybe you can try posting your blots and more people can help?
Posted 29 March 2010 - 06:49 PM
For PARP it is easier to see the larger cleaved form at 89kDa than the smaller 24kDa band.
I would also run a positive control on your gels, maybe your cells treated with an apoptotic inducer such as STS or etoposide to show caspase-3 and PARP cleavage?