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BSA as qPCR enhancer


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3 replies to this topic

#1 anacm

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Posted 17 March 2010 - 11:47 AM

I have to prepare a homemade mastermix for qPCR. Does BSA from NEB work well for this purpose?

Thanks in advance.

Ana :lol:

#2 Maddie

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Posted 24 March 2010 - 06:08 AM

I can't talk about NEB because I use SIGMA :P .
I can tell you SIGMA works really well though.
Theory is when we know everything and nothing is working. Practice is when everything is working and nobody knows why. Here, we combine theory and practice. Nothing is working and nobody knows why.

A. Einstein

#3 anacm

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Posted 25 March 2010 - 06:58 AM

Thanks for your answer!
Which Sigma BSA are you using? there are lots!

#4 Maddie

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Posted 26 March 2010 - 08:33 AM

It depends how much you need. I need a lot so I have to use the non acetylated ones.
You can buy them as powder or liquid and they work great.
Theory is when we know everything and nothing is working. Practice is when everything is working and nobody knows why. Here, we combine theory and practice. Nothing is working and nobody knows why.

A. Einstein




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