Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

Does anyone use potassium acetate to precipitate protein?

  • Please log in to reply
1 reply to this topic

#1 yja97



  • Active Members
  • Pip
  • 12 posts

Posted 17 March 2010 - 07:35 AM

Hi. I found that using salt and potassium acetate is fast and efficient to concentrate my protein samples. These solutions are usually used in DNA extraction.
These proteins samples are good and nice for SDS-PAGE and Western. But the problem is that these precipitate are not soluble in most buffers.
Does anybody used these solutions and succeed to solubilize the precipitates? My samples are really precious. I don't waste them.
Thank you in advance.

#2 Curtis


    Metaller Scientist

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 1,186 posts

Posted 17 March 2010 - 10:46 AM

I used aceton last time to precipitate protein and re-add less RIPA to have higher conc. It worked fine for me.

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.