What could be the cause for this GFP expression pattern...?
Posted 17 March 2010 - 07:30 AM
But when I transfect cells with my construct (a GFP tagged version at c-ter of my protein of interest, which I suspect to interact with a membrane receptor and is predicted to be citoplasmatic in in silico studies), it seems to have a bright punctate pattern (perhaps compatible with localization at membrane microdomains) along with a quite intense fluorescene in citoplasm and nucleus!!!
Should I suspect an artifactual localization of my fusion protein? Why I found it in the nucleus?
Any suggestion on how I must continue these experiments is welcome.
Posted 17 March 2010 - 03:31 PM
Posted 18 March 2010 - 07:06 PM
Posted 23 March 2010 - 12:01 AM
Posted 25 March 2010 - 01:13 PM
Posted 25 March 2010 - 01:19 PM