I've been trying to do a multiplex PCR by reading the plates at 2 different temperatures (they correspond to the Tm's of my products).l Earlier I was using the MJ research cycler with Opticon 2 which allowed me to obtain Ct values at both temperatures. Recently though, this instrument is down and I have to use one of the ABI real time machines. The problem with ABi 7000-7500 is that the software doesn't allow you to get multiple data collection points. Are there any other instruments besides BioRad cyclers that do this? I would appreciate the info very much. Thanks in advance!
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Help with multiplex PCR
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