Part of my mouse protocol (IACUC approved) involves euthanasia by carbon dioxide inhalation to collect tissues. I recently decided to add an assay that requires a small (<20microliters) of serum.
I don't really want to submit another IACUC addendum just to get permission to do live blood collection.
So my question is: How can I get around 100microliters of blood from a mouse that has very recently been euthanized?
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#2
mdfenko
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Posted 16 March 2010 - 10:32 AM
there should be some left in the heart.
talent does what it can
genius does what it must
i do what i get paid to do
genius does what it must
i do what i get paid to do
#3
wayne
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Posted 16 March 2010 - 03:12 PM
So I did a trial run today. I nicked the liver and a gush of blood came out. I pipetted around 150microliters of whole blood. Plenty of serum.
This round's on me!
This round's on me!
#4
Doki
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Posted 16 March 2010 - 05:33 PM
if U are already opening up the abdomen, why not cut the vena cava/aorta with scissors. U will get plenty of blood there. Or, I have not tried but U might be able to pull some out with a syringe after U expose these vessels is U want uncontaminated sample.
Simple living, highnot thinking
