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Has anyone worked with pCHF3


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#1 Anuradha

Anuradha

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Posted 16 March 2010 - 09:12 AM

Hey

I have to clone a microRNA gene that I have isolated from Brassica juncea into the plant transformation vector pCHF3 and mis-express it in Arabidopsis. I have no option of other plant transformation vector. I couldn't achieve the cloning of my insert in the vector.

Can someone suggest what ligation strategies can be used :)




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