Posted 15 March 2010 - 07:57 PM
In the textbook, it usually requires to autoclave medium 15 min, 15psi, 120 degrees.
While I think it depends on the volume of medium autoclaved. But what is the boundary for the time? If the bottle or flask contains only 1/3 volume medium, we can just autoclave 15min, is that right? How about 1/2 volume medium?
Thank you very much!
Posted 15 March 2010 - 10:59 PM
The pressure 15psi, 121C and 15 minutes is needed to destroy all the living organism (with the exception of strain 121). It doesn't matter if you are half bottle full or full bottle.
..."best of our knowledge, as far as we know this had never been reported before, though I can't possible read all the published journals on earth, but by perform thorough search in google, the keywords did not match any documents"...
"what doesn't kill you, makes you stronger"---Goddess Casandra reminds me to be strong
"It's all just DNA. Do it."---phage434
Posted 15 March 2010 - 11:47 PM
Posted 16 March 2010 - 09:31 AM
Posted 16 March 2010 - 09:48 AM