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how long to autoclave the medium


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#1 Rachel2010

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Posted 15 March 2010 - 07:56 PM

Hi,
In the textbook, it usually requires to autoclave medium 15 min, 15psi, 120 degrees.

While I think it depends on the volume of medium autoclaved. But what is the boundary for the time? If the bottle or flask contains only 1/3 volume medium, we can just autoclave 15min, is that right? How about 1/2 volume medium?

#2 TimGG

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Posted 15 March 2010 - 10:24 PM

Oh well, it really depends on your medium, some of the medium I am using are not suitable for autoclave so I filter it with a 0.2um filter.

anyway, 15 mins for 121C is the minumum in my lab~~ no matter of the volume, I hope it helps

#3 rhombus

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Posted 16 March 2010 - 05:31 AM

Hi,
In the textbook, it usually requires to autoclave medium 15 min, 15psi, 120 degrees.

While I think it depends on the volume of medium autoclaved. But what is the boundary for the time? If the bottle or flask contains only 1/3 volume medium, we can just autoclave 15min, is that right? How about 1/2 volume medium?



Dear Rachel 2010,

Cell Culture Media should never be autoclaved. It should always be filtered sterilised through 0.22uM filter (at worst). This is how the commercial suppliers do it

Bacterial Media is slightly different and CAN be autoclaved. It can also be filter sterilised. However autoclaving is always a cheaper option.

Kindest regards

Rhombus




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