Hi there, I am currently treating cells with drug dissolved in DMSO. When treating with corresponding serial dilutions of DMSO, I am consistently getting higher cell death in cells treated with lower DMSO vs higher amounts of DMSO. Does anyone have any reasons why this is happening? Thanks
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TimGG
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Posted 15 March 2010 - 10:35 PM
Hi Canpat,
Your simple question can have a very complex answer, with limited information, it is no way to have a definate answer, but "how high" concentration is high?? In my experience I always keep my DMSO at 2% (v/v) level no matter of the dilution. At low level of treatment sometimes does have a lower cell death then the negative control (DMSO), I guess you can try to expend your concentration range to a higher level to see what happen.
Or your drug might prevent cell death?! Good luck!
Your simple question can have a very complex answer, with limited information, it is no way to have a definate answer, but "how high" concentration is high?? In my experience I always keep my DMSO at 2% (v/v) level no matter of the dilution. At low level of treatment sometimes does have a lower cell death then the negative control (DMSO), I guess you can try to expend your concentration range to a higher level to see what happen.
Or your drug might prevent cell death?! Good luck!
