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How to Treat Your Samples for ELISA Test


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#1 Lulucy

Lulucy

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Posted 14 March 2010 - 06:01 PM

1. Supernatant of Cell Culture:
The cell culture should be kept at room temperature. After 10 min 1000g centrifugal, you can collect supernatant. Later on, sample should be kept at -20℃ and avoid repeat freezing.
2. Blood Serum:
Please keep sample around room temperature for 2 hours or keep it at 4℃ for a whole night. After that, you can centrifugal 20 min at 1000g, 4℃. Then, collect supernatant for test. You also can keep the sample at -20℃ and avoid repeat freezing.
3. Blood Plasma:
You can collect EDTA or heparin as the sample of gelation inhibitor. After its collection, it should centrifugal at 1000g, 2-8℃ for 15 min. you should keep the sample at -20℃ and avoid repeat freezing.
4. Urine:
Urine can be use for determine after asepsis collection and sediment removed. If you want to maintain urine for a long time, you can add 0.05% NaN3 at 4-8 cent great. Besides, you can add urine cryoprotectant and keep it at -20℃ too.
5. Tissue Homogenate:
At first, wash the tissue sample by PBS and remove extra blood. After homogenate, sample should be kept in PBS for a whole night at -20℃. After second freezing and 5000g centrifugal 5 min at 4℃,you can test it immediately.
In order to avoid toxic substance harm researcherís health, suitable protection methods should be follow during the operation.
Please read the manual carefully before using the kit.

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