Western Blot Antibodies for inhibiting protein activity??
Posted 14 March 2010 - 01:33 AM
I was wondering if we can use the antibodies that are used in western blotting to block the protein in cell culture and study its effects?
I want to inhibit a protein of interest, so i was thinking to use antibodies used in western blots to block it and then do a proliferation assay (or other assays) on it.
Please tell me if this is possible.. Many Thanks!!!
Posted 14 March 2010 - 06:04 PM
Most antibodies do not block protein function. They bind to other than the active parts of the protein. I know, however, that there's an antagonistic c-Kit antibody (Ack2), that blocks c-Kit signaling in vivo and in vitro (Disclaimer: our lab gets royalties for Ack2).
I'd recommend to check the literature carefully if there's any blocking antibody described for your protein. If it's a ligand/receptor pathway, you could also consider creating an Fc chimeric protein. But I'm not an expert for that...
Posted 16 March 2010 - 03:19 PM