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DNA size markers run at different levels in the same gel


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#1 lotus

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Posted 12 March 2010 - 02:59 PM

Hello,
I made a 1% agarose gel and loaded the dna ladders that we use in our lab for comparison. I loaded 2 aliquots of the 1 kb ladder in two lanes. There is as much as 0.5 kb difference in their positions on the gel. When compared to other ladders, there is a bigger difference. Is this normal? Which ladder do I trust i.e. which ladder truly depicts the correct size distribution of DNA on the gel?

#2 merlav

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Posted 15 March 2010 - 11:53 AM

Check the wire of the chamber, sometimes when is bend the samples don't electrophoresed equal distance
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#3 gebirgsziege

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Posted 16 March 2010 - 12:04 AM

did you pour your gel evenly? I mean is the thickness of the gel the same at both sides of the gel? Did you mix the agarose properly after dissoving or is it possible that you got a concentration gradient within the gel? and did the gel dissolve properly (if you are re-using gels)?
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