DNA size markers run at different levels in the same gel
Posted 12 March 2010 - 02:59 PM
I made a 1% agarose gel and loaded the dna ladders that we use in our lab for comparison. I loaded 2 aliquots of the 1 kb ladder in two lanes. There is as much as 0.5 kb difference in their positions on the gel. When compared to other ladders, there is a bigger difference. Is this normal? Which ladder do I trust i.e. which ladder truly depicts the correct size distribution of DNA on the gel?
Posted 15 March 2010 - 11:53 AM
I am among those who think that science has great beauty. A scientist in his laboratory is not only a technician: he is also a child placed before natural phenomena which impress him like a fairy tale.
Posted 16 March 2010 - 12:04 AM