3 replies to this topic

[aidaelshaer]

Dear Drs,

i have competent e-coli top 10, saved in liquid nitrogen from more than a year ago.
i think that it is no longer competent.
is it right?
if so , i will need to make it competent again,
So how can i prepare competent cells from frozen competent cells before a year in LN2?
i only need to to how to right begin.
Thanks a lot .
Aida

[almost a doctor]

aidaelshaer, on Mar 12 2010, 03:54 PM, said:

Dear Drs,

i have competent e-coli top 10, saved in liquid nitrogen from more than a year ago.
i think that it is no longer competent.
is it right?
if so , i will need to make it competent again,
So how can i prepare competent cells from frozen competent cells before a year in LN2?
i only need to to how to right begin.
Thanks a lot .
Aida

http://www.protocol-online.org/prot/Molecu...Cell/index.html

[phage434]

I suspect your cells are still highly competent, if they started out being competent.

You can make more by growing up a culture from your existing stock and following the protocol here, which is the one I use, after trying them all:
http://openwetware.o...competent_cells

[arera]

in my experience its still working..