Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

frozen competent cells


  • Please log in to reply
3 replies to this topic

#1 aidaelshaer

aidaelshaer

    member

  • Active Members
  • Pip
  • 12 posts
0
Neutral

Posted 12 March 2010 - 07:54 AM

Dear Drs,

i have competent e-coli top 10, saved in liquid nitrogen from more than a year ago.
i think that it is no longer competent.
is it right?
if so , i will need to make it competent again,
So how can i prepare competent cells from frozen competent cells before a year in LN2?
i only need to to how to right begin.
Thanks a lot .
Aida

#2 almost a doctor

almost a doctor

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 244 posts
15
Good

Posted 12 March 2010 - 07:57 AM

Dear Drs,

i have competent e-coli top 10, saved in liquid nitrogen from more than a year ago.
i think that it is no longer competent.
is it right?
if so , i will need to make it competent again,
So how can i prepare competent cells from frozen competent cells before a year in LN2?
i only need to to how to right begin.
Thanks a lot .
Aida



http://www.protocol-...Cell/index.html

#3 phage434

phage434

    Veteran

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 2,465 posts
247
Excellent

Posted 12 March 2010 - 08:25 PM

I suspect your cells are still highly competent, if they started out being competent.

You can make more by growing up a culture from your existing stock and following the protocol here, which is the one I use, after trying them all:
http://openwetware.o...competent_cells

#4 arera

arera

    member

  • Active Members
  • Pip
  • 18 posts
2
Neutral

Posted 25 May 2010 - 06:12 PM

in my experience its still working..




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.