3 replies to this topic

[niveda]

hi all,
I' m currently working on cardiac stem cells from adult and neonatal mice. I have tried isolating the c kit cells by primary cell culture following Anversa's method. Briefly I' m culturing the cardiac tissue explants in IMDM medium supplemented with 10% FBS,0.1mM mercaptoethanol, 100U penicillin  and 100 microgram/ ml streptomycin.
       But the facs analysis shows very less no of cells . Can anyone give me a suggestion on how to enrich my cell population.
I will be happy to have any simple suggestion,as i am totally blocked , and dont know how to proceed, what to do?
thank you in advance

[Hodag]

niveda, on Mar 11 2010, 12:16 AM, said:

I' m currently working on cardiac stem cells from adult and neonatal mice.

What criteria are you using to decide if something is a cardiac stem cell or something else?  Which specific CD markers are you looking at when you run your FACS analysis?

[niveda]

Hodag, on Mar 11 2010, 10:29 AM, said:

niveda, on Mar 11 2010, 12:16 AM, said:

I' m currently working on cardiac stem cells from adult and neonatal mice.

What criteria are you using to decide if something is a cardiac stem cell or something else?  Which specific CD markers are you looking at when you run your FACS analysis?

thankyou for your query, iam selecting the cells based on CD117 or c kit.

[Hodag]

niveda,

Check your private messges.