I' m currently working on cardiac stem cells from adult and neonatal mice. I have tried isolating the c kit cells by primary cell culture following Anversa's method. Briefly I' m culturing the cardiac tissue explants in IMDM medium supplemented with 10% FBS,0.1mM mercaptoethanol, 100U penicillin and 100 microgram/ ml streptomycin.
But the facs analysis shows very less no of cells

I will be happy to have any simple suggestion,as i am totally blocked

thank you in advance