How to visualize tumor cells in mice with out sacrificing the animals
Posted 10 March 2010 - 02:12 PM
Posted 10 March 2010 - 03:44 PM
They also sell different luciferase expressing cell lines for in vivo study. http://www.caliperls...-animal-models/
You can find protocols here http://www.protocol-...ging/index.html
Posted 22 July 2010 - 10:01 AM
The invivoimagingsolutions.com site provides reagents, products and instrumentation related to in vivo imaging. They have readymade luc2, GFP or custom lentiviruses to transfect cell lines and offer custom cell transfection.
Posted 29 July 2010 - 05:47 AM
I have no experience with animal work so far. We are thinking about doing some xenograft studies by transplanting some lymphoma cells derived from human patients into mice. We'd like to visualize the cells in live animals by expressing a luciferase gene or GFP gene in the cells. Could anybody recommend a few papers that have some introduction and detailed description of this type of techniques? Thanks!
Maybe you could check out these- 1 article and 2 reviews about bioluminescence imaging
A luciferase transgenic mouse model: visualization of prostate development and its androgen responsiveness in live animals (C-L Hsieh et al., 2005; Journal of Molecular Endocrinology)
Bioluminescence Imaging (Ruxana T. Sadikot and Timothy S. Blackwell, 2005; PROCEEDINGS OF THE AMERICAN THORACIC SOCIETY)
Bioluminescence imaging in living organisms (David K Welsh and Steve A Kay, 2005; Current Opinion in Biotechnology)