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cell count and dilution


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#1 jasmine0507

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Posted 10 March 2010 - 12:07 PM

Hi all,

I have 4 x 10^4 cells/ml and 5 ml of culture and I want to put 5 x 10^4 cells per well in a 24-well plate.

Can anyone explain me how to calculate this ? and total solution i need to prepare for 24 wells.

another question : If I want 10^5 cells at 60-70 % confluency, will it be ok to seed 50,000 cells 18-24h before ?

Thank you

#2 bob1

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Posted 10 March 2010 - 03:11 PM

Use the same equations you would use for making and diluting any other solutions c=n/v and Ci*Vi=Cf*Vf.

There are a lot of threads on counting and diluting cells in the cell culture sub-forum.




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