Does anyone know if:
- increasing the MOI of shRNA virus infection increases the knockdown effectiveness in target cells?
- infecting target cells multiple time increases knockdown effectiveness?
- after infecting with a shRNA lentivirus carrying a GFP reporter, is the level of GFP expression proportional to the knockdown effectiveness i.e. if all the target cells are GFP positive, will FACS sorting for the top 5% GFP intensity give me a population of cells with better knockdown?
Thanks,
Sam
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Effect of MOI on lentiviral shRNA knockdown
Started by saml, Mar 10 2010 10:31 AM
1 reply to this topic
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Posted 12 March 2010 - 05:36 PM
Does anyone know if:
- increasing the MOI of shRNA virus infection increases the knockdown effectiveness in target cells?
YES, but not always true
- infecting target cells multiple time increases knockdown effectiveness?
YES, but not always true
- after infecting with a shRNA lentivirus carrying a GFP reporter, is the level of GFP expression proportional to the knockdown effectiveness i.e. if all the target cells are GFP positive, will FACS sorting for the top 5% GFP intensity give me a population of cells with better knockdown?
YES, ideally top 5% GFP-cells harbor more copies of lentivirus
- increasing the MOI of shRNA virus infection increases the knockdown effectiveness in target cells?
YES, but not always true
- infecting target cells multiple time increases knockdown effectiveness?
YES, but not always true
- after infecting with a shRNA lentivirus carrying a GFP reporter, is the level of GFP expression proportional to the knockdown effectiveness i.e. if all the target cells are GFP positive, will FACS sorting for the top 5% GFP intensity give me a population of cells with better knockdown?
YES, ideally top 5% GFP-cells harbor more copies of lentivirus
