Plasmid frameshift problem after clonning
Posted 10 March 2010 - 10:08 AM
I've got a v. basic clonning question here. I've inserted a full-length gene into a pET15b vector. When I got my sequencing results, I found the reading frame of the vector is changed. The plasmid amino acid sequence would be changed upon clonning of my insert in. I'm wondering if this frameshift of the vector dose matter or not?
I'm very new to this, hope someone could help.
Posted 10 March 2010 - 03:16 PM
Posted 10 March 2010 - 06:30 PM