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Best methods for storing cDNA


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#1 phosphate girl

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Posted 09 March 2010 - 08:09 AM

Hello everyone,

Am currently making cDNA for qPCR. I will need to store it for a week or two, what is the best way to store it? Is it OK to store it in water or should I be using a buffer solution? Any suggestions ?

#2 Vini

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Posted 09 March 2010 - 09:02 AM

Hello everyone,

Am currently making cDNA for qPCR. I will need to store it for a week or two, what is the best way to store it? Is it OK to store it in water or should I be using a buffer solution? Any suggestions ?



its ok to store it in water at -20 degrees.

#3 phosphate girl

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Posted 11 March 2010 - 03:01 AM

its ok to store it in water at -20 degrees.
[/quote]

Great Thanks - is it OK to store it in the buffer it was synthesized in or do you add TE or Tris buffer? I want to do qPCR with it but not straight away (am very new to molecular biology). I want to make sure it doesn't degrade prior to the qPCR.

Thanks :rolleyes:

#4 merlav

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Posted 15 March 2010 - 11:58 AM

For QPCR is better to dilute all in water, sometimes an excess of salts could make that the reaction don't function as expected.
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I am among those who think that science has great beauty. A scientist in his laboratory is not only a technician: he is also a child placed before natural phenomena which impress him like a fairy tale.
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