Hello everyone,
Am currently making cDNA for qPCR. I will need to store it for a week or two, what is the best way to store it? Is it OK to store it in water or should I be using a buffer solution? Any suggestions ?
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#2
Vini
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Posted 09 March 2010 - 09:02 AM
phosphate girl, on Mar 9 2010, 09:39 PM, said:
Hello everyone,
Am currently making cDNA for qPCR. I will need to store it for a week or two, what is the best way to store it? Is it OK to store it in water or should I be using a buffer solution? Any suggestions ?
Am currently making cDNA for qPCR. I will need to store it for a week or two, what is the best way to store it? Is it OK to store it in water or should I be using a buffer solution? Any suggestions ?
its ok to store it in water at -20 degrees.
#3
phosphate girl
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Posted 11 March 2010 - 03:01 AM
its ok to store it in water at -20 degrees.
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Great Thanks - is it OK to store it in the buffer it was synthesized in or do you add TE or Tris buffer? I want to do qPCR with it but not straight away (am very new to molecular biology). I want to make sure it doesn't degrade prior to the qPCR.
Thanks
[/quote]
Great Thanks - is it OK to store it in the buffer it was synthesized in or do you add TE or Tris buffer? I want to do qPCR with it but not straight away (am very new to molecular biology). I want to make sure it doesn't degrade prior to the qPCR.
Thanks
#4
merlav
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Posted 15 March 2010 - 11:58 AM
For QPCR is better to dilute all in water, sometimes an excess of salts could make that the reaction don't function as expected.
Science without religion is lame, religion without science is blind.
Albert Einstein
I am among those who think that science has great beauty. A scientist in his laboratory is not only a technician: he is also a child placed before natural phenomena which impress him like a fairy tale.
Marie Curie
Albert Einstein
I am among those who think that science has great beauty. A scientist in his laboratory is not only a technician: he is also a child placed before natural phenomena which impress him like a fairy tale.
Marie Curie
