colony formation assay
Posted 09 March 2010 - 07:39 AM
I need to perform colony transfection assay follwing transfection of my gene of interest (GOI). After transecting my cells for 24hrs, how many cells do I seed in a 6 well plate for colony formation? 500 or 2000? Any ideas? I plated 15000 cells over 2 weeks and it was not possible to conunt them after crystal violet staining. Any recommendations to the procol will be appreciated.
Posted 09 March 2010 - 09:32 AM
Posted 09 March 2010 - 09:10 PM
Thank you for the suggestion. I'm working with liver cancer cell lines too and I'm planning to seed PLC and 97L cells, both of which grow fast and thus 500 cells should be good.