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How to improve lentiviral transduction efficiency in mouse cell lines


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#1 chandan

chandan

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Posted 09 March 2010 - 04:04 AM

I am trying to transduce 3T3 cell line using VSV-G and CMV with turboGFP. When I transduce my cells, I get zero percent transduction. Where as, in my control, (which i know, it is positive control in Human Mesenchymal stem cells) i get around 90 transduction efficiency.

Is there any way, i am improve transduction efficiency in rodent cells?
I followed this paper for answer.
http://www.nature.co...l/3301708a.html

Thanks in advance,
Chandan

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