Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Biotinylated!


  • Please log in to reply
2 replies to this topic

#1 edrummond

edrummond

    member

  • Members
  • Pip
  • 2 posts
0
Neutral

Posted 09 March 2010 - 02:03 AM

Hi,

I'm new to this area and need a clear definition of what it means when a primer is "biotinylated"...is it anything to do with the vitamin Biotin? And its affinity for binding with streptavidin?

Edited by edrummond, 09 March 2010 - 02:31 AM.


#2 phage434

phage434

    Veteran

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 2,476 posts
251
Excellent

Posted 09 March 2010 - 06:33 AM

Yes, indeed it is the same biotin. In primers it is typically attached to a relatively long linker, c12 or c15 at the 5' end of oligos to make them visible in southern blots when detected by enzyme linked streptavidin. They can also be used to immobilize oligos on beads or surfaces, and to selectively remove biotinylated fragments from solution.

#3 Shifting Reality

Shifting Reality

    member

  • Active Members
  • Pip
  • 24 posts
0
Neutral

Posted 04 August 2014 - 03:14 AM

Hello,

 

I've made a little research about streptavidin-coated magnetic beads. I would like to immobilize biotinylated PCR products. I have 3 candidate products. Theoretically, all of these items are ideal for this purpose.

 

There is huge price difference between these products (and some differences in certain parameters).

 

- Dynabeads® M-280 Streptavidin ; bead diameter: 2,8 um ; Binding Capacity (free biotin): 650-900 pmoles/mg beads ; concentration: 10 mg/ml ; 10 ml : 1 500 $

 

- solulink ; bead diameter: 0,8 um ; Binding Capacity (free biotin):  > 14 nmol/mg ; concentration: 10 mg/ml ; 10 ml: 800 $

 

spherotech ; bead diameter: 0,5 - 4.5 um ; Binding Capacity (free biotin): no described ; concentration: 0,5 - 1 w/v % ; 10 ml: 200-250 $

 

But I don't know what so special in M-280 making it very expensive. smile.png

 

Can anybody tell me what I should choose ?

 

Thanks for the answer ! smile.png






Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.