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Hek293 at room temperature for assay


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#1 Buddy

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Posted 08 March 2010 - 01:53 PM

Hi,
I am trying to study a drosophila temperature-sensitive transposition system in human 293 cells. The Drosophila transposase is active at 25C, but I normally culture and grow 293 cells at 37C. Does anyone have any experience or know of a someone who has cultured 293 at RT or at temperatures cooler than 37C?

Thanks,
Buddy

#2 bob1

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Posted 08 March 2010 - 03:24 PM

They should still grow, just slower than they would at 37.




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