Primaria/gelatin plates and gravity-defying fixed cells
Posted 08 March 2010 - 09:17 AM
The problem I am having is during the cell scraping, the cells love to 'float' up the dish, defying gravity and working their way up any residual liquid across the surface of the dish. I don't know if this is specifically due to charge from the Primaria surface treatment or some other reason. This problem makes it very difficult to collect all the cells, even after three or four sequential rinses with fresh PBS.
Has anyone experienced this problem with NRVMs, or Primaria/gelatin plates in general? If anyone has any advice it would be much appreciated!
Posted 15 March 2010 - 09:35 AM
Posted 07 July 2010 - 08:47 AM